PLACENTAL SECRETORY FACTORS INFLUENCE TO THP-1 CELLS PHENOTYPE AND THP-1 CELLS TRANSENDOTHELIAL MIGRATION

-retinoic acid in undifferentiated THP-1 cells

We have previously shown that acyl-coenzyme A: cholesterol acyltransferase-1 (ACAT-1) protein content increases significantly during the human monocyte-macrophage differentiation process. To gain further insight, we used undifferentiated human monocytic THP-1 cells as a model system with which to examine whether ACAT-1 mRNA and protein content can be increased by treating cells with 1,25-dihydr...

Mono-ethylhexyl phthalate stimulates prostaglandin secretion in human placental macrophages and THP-1 cells

BACKGROUND Diethylhexyl phthalate (DEHP) is widely used as a plasticizer in polyvinyl chloride products. DEHP exposure, which is widespread in the US, increases preterm birth risk; however, the mechanisms driving this relationship are unclear. Because cyclooxygenase-2 (COX-2) dependent prostaglandin synthesis is implicated in preterm birth, we evaluated effects of mono-2-ethylhexyl phthalate (M...

Processing of proteoglycans after uptake by THP-1 cells.

Efficient non-viral transfection of THP-1 cells.

Macrophages are an important part of the cellular immune system and play a key role during immune responses. Thus, macrophages are interesting targets in basic and clinical research. Primary monocytes or monocyte-derived macrophages do not proliferate on a suitable scale so that their use for functional studies in vitro is limited. Immortal proliferating cell lines, such as the human THP-1 mono...

Cytotoxic effects of TEGDMA on THP-1 cells in vitro.

OBJECTIVE Resin based dental materials are not stable in the oral environment and may release their components into biological media. These components may include substances such as triethylene glycol dimethacrylate (TEGDMA), which is a major co-monomer of dental resin materials. This release can trigger host immune and inflammatory responses against foreign materials, mediated by monocytes. Th...